https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39306 +/sugar antiporters MdTST1 and MdTST2. Overexpression of MdERDL6-1 unexpectedly increased not only glucose (Glc) concentration but also that of fructose (Fru) and sucrose (Suc) in transgenic apple and tomato leaves and fruits. RNA sequencing (RNA-seq) and expression analyses showed an up-regulation of TST1 and TST2 in the transgenic apple and tomato lines overexpressing MdERDL6-1. Further studies established that the increased sugar concentration in the transgenic lines correlated with up-regulation of TST1 and TST2 expression. Suppression or knockout of SlTST1 and SlTST2 in the MdERDL6-1–overexpressed tomato background reduced or abolished the positive effect of MdERDL6-1 on sugar accumulation, respectively. The findings demonstrate a regulation of TST1 and TST2 by MdERDL6-1, in which Glc exported by MdERDL6-1 from vacuole up-regulates TST1 and TST2 to import sugars from cytosol to vacuole for accumulation to high concentrations. The results provide insight into the regulatory mechanism of sugar accumulation in vacuoles mediated by the coordinated action of two classes of tonoplast sugar transporters.]]> Wed 10 Aug 2022 11:02:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47077 Tue 13 Dec 2022 16:21:31 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37236 Tue 10 Nov 2020 15:18:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1116 Sat 24 Mar 2018 08:32:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20431 Sat 24 Mar 2018 08:03:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21684 Mon 23 Nov 2015 12:25:08 AEDT ]]>